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Laboratory Manual of Microbiology (Record no. 17194)

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000 -LEADER
fixed length control field	06782nam a22001817a 4500
003 - CONTROL NUMBER IDENTIFIER
control field	OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field	20240612124606.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field	211020b xxu\|\|\|\|\| \|\|\|\| 00\| 0 eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number	978-93-86102-53-9
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title	ENGLISH
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number	576 KUM
100 ## - MAIN ENTRY--PERSONAL NAME
Author name	Kumar, Vivek
245 ## - TITLE STATEMENT
Title	Laboratory Manual of Microbiology
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc.	Jodhpur
Name of publisher, distributor, etc.	Scientific Publisher
Date of publication, distribution, etc.	2019
300 ## - PHYSICAL DESCRIPTION
Page	215
500 ## - GENERAL NOTE
General note	Description:<br/>This laboratory manual of microbiology has been written to meet the needs of students taking microbiology as major or subsidiary subject. The intention is to provide the students with well organized, user-friendly tool to better enable them to understand laboratory aspects of microbiology as well as to hopefully make learning laboratory material and preparing for independent player of a given experiment. Each exercise provides step-by-step procedure to complete the assignment successfully and easily. The lab exercises are designed to give the student hands-on laboratory experience to better reinforce certain topics discussed in exercise. The glossary is included covering terms as well as basic, discipline-specific terminology from microbiology that will be helpful to its readers. The main contents of the manual are: Microbiology laboratory practices and safety rules, Basic laboratory techniques, Microscopy, Staining and motility techniques, Environmental microbiology, Microbiological culture techniques, Growth of lactose fermenting and non fermenting microbes, Medical microbiology, Environmental effect on bacterial growth, Application of microbiology, Microbiology of milk and Appendices. The academic level of the book is graduate, post graduate students, research workers, teachers and scientists dealing with basic and applied aspects of microbiology.<br/><br/>Contents:<br/>1 Microbiology laboratory practices and safety rules<br/>I BASIC LABORATORY TECHNIQUES<br/>2. Media preparation<br/> 2.1. Sub culturing or picking off:<br/> 2.2. Broth cultures<br/>3. Sterilization and disinfection<br/>4. Inoculation and other aseptic procedures<br/> 4.1. Using a wire loop<br/> 4.2. Flaming procedure<br/> 4.3. Using a pipette<br/> 4.4. Flaming the neck of bottles and test tubes<br/>5. Inoculation using a pipette<br/>6. Pouring the media in Petri plate<br/>7. Use of spreader<br/>8. Spread plate technique <br/>II MICROSCOPY<br/>9. Use and care of microscope<br/>10. Dark field microscopy<br/> 10.1. Bright field microscopy<br/> 10.2. Resolving power of microscope<br/> 10.3. Phase contrast field microscopy<br/> 10.4. Fluorescence microscopy<br/> 10.5. Electron microscopy<br/>11. Ocular micrometery<br/>III STAINING AND MOTILITY TECHNIQUES <br/>12. Staining preparations<br/>13. Smear making<br/>14. Simple staining<br/>15. Gram staining of bacteria<br/>16. Capsule staining of bacteria<br/>17. Capsule staining by negative, indirect or background staining <br/>18. Acid fast (Ziehl-Neelsen) staining of bacteria<br/>19. Endospore staining of bacteria<br/>20. Dorner’s method of endospore staining<br/>21. Bacterial flagella staining<br/>22. Viability staining technique of bacteria<br/>23. Lactophenol cotton blue staining of fungi<br/>24. Staining of Vesicular Arbuscular Mycorrhiza (VAM)<br/>25. Staining of actinomycetes culture<br/>26. Hanging drop slide for bacterial motility<br/>27. Motility Determination (Tube method)<br/>28. Slide technique for protozoa motility<br/>IV BIOCHEMICAL ACTIVITIES OF MICROBES<br/>29. Urease activity of bacteria<br/>30. Nitrate reduction test<br/>31. Catalase test<br/>32. Lipid hydrolysis test<br/>33. Oxidase test<br/>34. DNase production test<br/>35. Starch hydrolysis test or amylase production test <br/>36. Gelatin hydrolysis or production of gelatinase<br/>37. IMVIC tests<br/> 37.1. Indole Test<br/> 37.2. Methyl Red and Voges-Proskauer Tests<br/> 37.3. Citrate Test<br/>38. Hydrogen sulfide production and motility<br/>39. TSI (Triple Sugar Iron) and KIA (Kligler's Iron Agar)<br/>40. Casein hydrolysis<br/>41. Estimation of phytohormone IAA production from bacteria<br/>42. Estimation of ammonia production by bacteria<br/>43. Solubilization of mineral phosphate by microbes<br/>V ENVIRONMENTAL MICROBIOLOGY<br/>44. Multiple tube fermentation tests<br/> 44.1. Presumptive coliform test<br/> 44.2. Confirmed coliform test<br/> 44.3. Completed coliform test<br/>45. Counting of Ascaris eggs in raw or treated sewage water<br/>46. Determination of dissolved oxygen (DO) of water<br/>47. Determination of biological oxygen demand (BOD) of water<br/>48. Determination of chemical oxygen demand (COD) of water<br/>49. Detection of coliforms in water using membrane filtration technique<br/>VI MICROBIOLOGAL CULTURE TECHNIQUES<br/>50. Single Colony Isolation<br/>51. Viable plate count or Pour plate or serial dilution agar technique<br/>52. Counting of soil microorganisms<br/>53. Isolation of Rhizobium from root nodules<br/>54. Testing of nodulation ability by Rhizobia<br/>55. Isolation of Azotobacter from soil<br/>56. Carrier material for biofertilizers<br/>57. Seed treatment with biofertilizers<br/>58. Selection of bio-control agent<br/>59. Isolation of pure culture and their preservation<br/>60. Cultivation of anaerobic bacteria<br/>VII GROWTH OF LACTOSE FERMENTING AND NON- FERMENTING MICROBES<br/>61. Growth of bacteria using selective and differential media<br/>62. EMB (Eosine methylene blue) agar for E. coli<br/>63. MacConkey agar for enterobacterieaceae<br/>64. Salmonella-Shigella (SS) agar for Salmonella and Shigella<br/>65. XLD (Xylose Lysine Deoxycholate) agar for enterobacteriaceae<br/>VIII MEDICAL MICROBIOLOGY<br/>66. Micro-flora of upper respiratory tract<br/>67. Enumeration of urine bacteria using pour plate technique<br/>68. Use of urine dip slide for bacteria examination<br/>69. Counting of bactiurea using calibrated loop method<br/>70. Widal test: The tube agglutination test<br/>71. Slide agglutination test for rapid diagnosis of pathogen<br/>72. The Ouchterlony technique (double immuno-diffusion method)<br/>73. Determination of Rh factor<br/>IX ENVIRONMENTAL EFFECT ON BACTERIAL GROWTH<br/>74. Effect of pH on bacterial growth<br/>75. Effect of oxygen on the growth of microorganisms<br/>76. Effect of temperature on growth of microorganisms<br/>77. Effect of osmotic pressure on growth of microorganisms<br/>78. Effect of radiation (Ultraviolet light) on growth of microorganism<br/>X APPLICATION OF MICROBIOLOGY<br/>79. Determination of viral plaque assay<br/>80. The one step bacteriophage growth curve<br/>81. Kirby-Bauer disk diffusion susceptibility test<br/>82. Determination of phenol coefficient of a chemical or effect of disinfectant on bacteria<br/>83. Determination of bacterial growth curve<br/>84. Demonstration of Koch's postulates<br/>85. Identifying Gram-negative enteric bacteria using API 20E system <br/>86. Identifying Gram-negative, oxidase negative enteric bacteria using Enterotube II system <br/>XI MICROBIOLOGY OF MILK<br/>87. Examination of milk quality by methylene blue reduction test<br/>88. Counting of number of bacteria in milk using Breed method
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Source of classification or shelving scheme	Dewey Decimal Classification
Koha item type	BOOKS

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Source of classification or shelving scheme	Not for loan	Collection code	bill no.	bill date	Home library	Current library	Date acquired	Source of acquisition	Coded location qualifier	Cost, normal purchase price	Total Checkouts	Full call number	Accession No	Date last seen	Price effective from	Koha item type	Public note	Date checked out
Dewey Decimal Classification	Not For Loan	Agricultur	NIPA/21-22/6196	30.03.2021	CENTRAL LIBRARY	CENTRAL LIBRARY	20/10/2021	New India Publishing Agency, New Delhi	REF	275.00		576 VIV	36374	20/10/2021	20/10/2021	BOOKS	Reference Books
Dewey Decimal Classification		Agricultur	NIPA/21-22/6196	30.03.2021	CENTRAL LIBRARY	CENTRAL LIBRARY	20/10/2021	New India Publishing Agency, New Delhi		275.00		576 VIV	36375	20/10/2021	20/10/2021	BOOKS
Dewey Decimal Classification		Agricultur	NIPA/21-22/6196	30.03.2021	CENTRAL LIBRARY	CENTRAL LIBRARY	20/10/2021	New India Publishing Agency, New Delhi		275.00	1	576 VIV	36376	04/01/2023	20/10/2021	BOOKS		24/11/2022