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Research Methodology in Plant Science (Record no. 17202)

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000 -LEADER
fixed length control field	14721nam a22002177a 4500
003 - CONTROL NUMBER IDENTIFIER
control field	OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field	20240612153636.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field	211020b xxu\|\|\|\|\| \|\|\|\| 00\| 0 eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number	978-93-89832-27-3
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title	ENGLISH
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number	574.028 NAR
100 ## - MAIN ENTRY--PERSONAL NAME
Author name	Narayana, P. Suresh
245 ## - TITLE STATEMENT
Title	Research Methodology in Plant Science
250 ## - EDITION STATEMENT
Edition statement	2nd
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc.	Jodhpur
Name of publisher, distributor, etc.	Scientific Publisher
Date of publication, distribution, etc.	2021
300 ## - PHYSICAL DESCRIPTION
Page	457
500 ## - GENERAL NOTE
General note	Description:<br/>The book comprises of different chapters associated with methodology in Plant Science (Botany), describing in a simple and comprehensive way. The importance of creativity and motivation in research, the planning and proposal of research project, the description of different scientific techniques involved in research are described in an elaborate way. It also includes the sources/collection of scientific information, method of scientific report/paper/thesis writing etc. The book is also a source of different aspects of research methodology tailored to the needs of postgraduate students/research scholars for easy understanding. The book is also profusely illustrated. Compared to the first edition, the entire text has been thoroughly revised and updated in addition to the inclusion of four new chapters (Recent Advances in Electrophoresis, Chromatography – Latest and Advanced Techniques, Polymerase Chain Reaction and Photon Counting and Photo-Multiplier Tubes). We hope this edition will be more useful to the readers and will continue to serve the purpose more effectively. <br/>Contents:<br/>1. RESEACH METHODOLOGY <br/><br/>Introduction <br/><br/>Research - What it is? <br/><br/>Aim and Purpose of Research <br/><br/>Early History of Research <br/><br/>Types of Research <br/><br/>Possible Motives for Doing Research <br/><br/>Methodology - What it is? <br/><br/>Types of Research Methodology <br/><br/>Research Design <br/><br/>Research Plan <br/><br/>Concepts and Context of Research <br/><br/>Research Logistics <br/><br/>Research Methods versus Research Methodology <br/><br/>Research Proposal <br/><br/>Different Stages of Research <br/><br/>Criteria for Good Research <br/><br/>Objectives of Research <br/><br/>Why do people carry out Research? <br/><br/>Importance of Research <br/><br/>Significance of Research <br/><br/>2. CENTRIFUGATION <br/><br/>Introduction <br/><br/>Centrifugation - Definition <br/><br/>Types of Centrifugation <br/><br/>Preparative Ultracentrifugation - Separation Methods<br/><br/>Centrifuge - The Instrument <br/><br/>Centrifuge - Components and Parameters <br/><br/>Centrifuge - Operation <br/><br/>Common Centrifuges Used in Biological Sciences <br/><br/>Centrifuges - Types <br/><br/>Centrifuges - Classification <br/><br/>Ultracentrifuge - Basic Principle <br/><br/>Preparative Centrifugation - Types of Rotors <br/><br/>Rotors - Selection <br/><br/>Rotors and Accessories - Care<br/><br/>Centrifuge - Containers <br/><br/>Density Gradient Separation - Formation and Choice of Density Gradients <br/><br/>Gradient Material - Nature and Use <br/><br/>Suitable Density Gradient Medium - Selection <br/><br/>Fractions - Collection <br/><br/>Sample Application to the Gradient <br/><br/>Recovery and Monitoring of Gradients <br/><br/>Analysis of Sub-cellular Fractions <br/><br/>Presentation of the Results <br/><br/>Analytical Ultracentrifuge - Applications <br/><br/>Centrifuges - Safety Aspects in Use <br/><br/>Centrifugation - Applications in Biological Research <br/><br/>Many Uses of Centrifuge <br/><br/>3. PLANT TISSUE CULTURE <br/><br/>Introduction <br/><br/>Plant Tissue Culture - Materials and Methods<br/><br/>Explant <br/><br/>Aseptic Environment <br/><br/>Tissue Culture Media or Nutrient Media <br/><br/>Nutritional Composition of Most Common Plant Cell/Tissue Culture Media (Angiosperms) <br/><br/>Composition or Components of Tissue Culture Media<br/><br/>General Technique of Plant Tissue Culture <br/><br/>Plant Tissue Culture Protocol (General) <br/><br/>Plant Tissue Culture - Laboratory and Equipment <br/><br/>Methodology for the Establishment of Cultures <br/><br/>Types of Plant Tissue Culture <br/><br/>Applications of Plant Tissue Culture<br/><br/>4. ELECTROPHORESIS <br/><br/>Introduction <br/><br/>Definition<br/><br/>How Separation Occurs in Electrophoresis? <br/><br/>Requirements for Electrophoresis <br/><br/>Basic Principles of Electrophoresis <br/><br/>Electrophoresis - Method<br/><br/>Factors that affect Electrophoresis <br/><br/>Physical Background of Electrophoresis <br/><br/>Instrumentation for Electrophoresis <br/><br/>Electrophoresis – Apparatus <br/><br/>Theory of Electrophoresis <br/><br/>Types of Electrophoresis <br/><br/>Specific Techniques of Electrophoresis Commonly Used in Biochemistry <br/><br/>Detection Methods – Blotting <br/><br/>Electrophoresis – Applications <br/><br/>RECENT ADVANCES IN ELECTROPHORESIS <br/><br/>Introduction <br/><br/>Non-linear Frictiophoresis or Non-linear Electrofrictiophoresis <br/><br/>ZIFE (Zero Integrated Field Electrophoresis) <br/><br/>Pulsed - field Gel Electrophoresis (PFGE) <br/><br/>Isotachophoresis (ITP) <br/><br/>Transient Isotachophoresis (tITP) <br/><br/>Multi-junction IEF System <br/><br/>Dielectrophoresis (DEP) <br/><br/>Optical Dielectrophoresis <br/><br/>Affinity Electrophoresis <br/><br/>Difference Gel Electrophoresis (DIGE) <br/><br/>Discontinuous Electrophoresis (colloquially Disc Electrophoresis) <br/><br/>Microfluidic Chip-based Electrophoresis <br/><br/>Iontophoresis <br/><br/>Temperature Gradient Gel Electrophoresis (TGGE) and Denaturing Gradient Gel Electrophoresis (DGGE) <br/><br/>Free Flow Electrophoresis (FFE) or Carrier-Free Electrophoresis <br/><br/>New Technique to Separate Microsatellite Alleles <br/><br/>Conclusion <br/><br/>5. HERBARIUM TECHNIQUE <br/><br/>Herbarium - What it is? <br/><br/>Plant Collection <br/><br/>Why Preserve Plant Specimens? <br/><br/>Preservation of the Plant Specimens <br/><br/>Equipment Needed for Collection of the Plant Specimens <br/><br/>Method to keep Wet Plant Material in Adverse Conditions <br/><br/>Methods of Drying the Plant Specimen <br/><br/>What Makes a Good Specimen? <br/><br/>The Data to be recorded in the Field Note Books <br/><br/>Mounting of the Dry Plant Specimens <br/><br/>Labeling the Plant Specimens <br/><br/>Filling or Arrangement of the Plant Specimens <br/><br/>Special Care of the Plant Specimens <br/><br/>Safe Storage of the Sheets / Specimens <br/><br/>Safe Preservation of the Specimens (Plants) <br/><br/>Use and Significance of the Herbarium<br/><br/>Role of Herbarium in Modern Plant Taxonomy <br/><br/>6. CHROMATOGRAPHY <br/><br/>Introduction <br/><br/>Definition <br/><br/>Principle <br/><br/>Chromatography - Early History <br/><br/>Classification of Chromatographic Methods <br/><br/>Special Techniques of Chromatography <br/><br/>Hyphenated (Hybrid) Techniques and Alternative Methods of Detection in Chromatography <br/><br/>Chromatography - Basic Principle <br/><br/>Types of Chromatography <br/><br/>Adsorption Chromatography <br/><br/>Partition Chromatography <br/><br/>Planar Chromatography <br/><br/>General Procedure - Paper Chromatography (PC) <br/><br/>Thin Layer Chromatography (TLC) <br/><br/>High Pressure Liquid Chromatography (HPLC) or High Performance Liquid Chromatography <br/><br/>General Procedure - High-Performance Liquid Chromatography (HPLC) <br/><br/>Chromatography - Applications <br/><br/>Chromatography - Applications in Biology and Chemistry <br/><br/>Chromatography - Uses in Pharmacognosy <br/><br/>Chromatography - Applications for the Analysis of Herbal Drugs <br/><br/>Chromatography - Latest and Advanced Techniques <br/><br/>Evolution of Chromatography <br/><br/>Chromatography - Improvements - Multi-dimensional Analysis <br/><br/>Dye-Ligand Chromatography <br/><br/>Hydrophobic Interaction Chromatography (HIC) <br/><br/>Pseudo-affinity Chromatography <br/><br/>Immobilized Metal Affinity Chromatography (IMAC) <br/><br/>Perfusion Chromatography (PC) <br/><br/>Supercritical Fluid Chromatography (SFC) <br/><br/>Expanded Bed Adsorption (EBA) Chromatography <br/><br/>Nanoflow Liquid Chromatography (NLC) <br/><br/>Micellar Liquid Chromatography (MLC) <br/><br/>Electrochromatography <br/><br/>Capillary Electrochromatography (CEC) <br/><br/>Weak Affinity Chromatography (WAC) <br/><br/>High Performance Liquid Chromatography (HPLC) <br/><br/>Conclusion <br/><br/>Hy-phe-nated Techniques or Hybrid Techniques <br/><br/>Hy-phe-nated HPLC (High-performance Liquid Chromatography) Methods <br/><br/>7. BOTANICAL MICRO-TECHNIQUE <br/><br/>Introduction <br/><br/>Killing and Fixation <br/><br/>Embedding the Material in Paraffin <br/><br/>Stains and Staining Methods <br/><br/>Procedure of Staining <br/><br/>Dehydration of the Material <br/><br/>Clearing the Sections <br/><br/>Dewaxing the Sections <br/><br/>Mounting the Material on to the Slide <br/><br/>Adhering Sections to the Slides <br/><br/>Ringing the Slide / Sealing the Cover slip <br/><br/>Slide Label - Preparation <br/><br/>Free Hand Sections or Sectioning by Hand <br/><br/>Free Hand Sectioning - Procedure <br/><br/>Epidermal Peeling - Preparation <br/><br/>8. MICROSCOPY <br/><br/>Introduction <br/><br/>Microscopy - Definition <br/><br/>Types of Microscopes <br/><br/>Optical (Light) Microscope <br/><br/>Electron Microscope <br/><br/>Scanning Electron Microscope (SEM) <br/><br/>Transmission Electron Microscope (TEM) <br/><br/>Scanning Probe Microscope (SPM) <br/><br/>Optical or Light Microscopy <br/><br/>The Compound Light Microscope <br/><br/>Electron Microscope <br/><br/>Cytophotometry or Microfluorometry <br/><br/>Phase Contrast Microscopy (PCM) <br/><br/>Fluorescence Microscopy (FM) <br/><br/>Scanning Electron Microscopy (SEM) <br/><br/>Transmission Electron Microscopy (TEM) <br/><br/>Electron Microscopy (EM) <br/><br/>9. SMEAR AND SQUASH TECHNIQUE <br/><br/>Introduction <br/><br/>Smear and Squash <br/><br/>Squash Technique for Root Tips - Protocol<br/><br/>Preparation of the Onion Root Tip Squash <br/><br/>Chromosome Squash Technique <br/><br/>Preparation of the Anther Smear <br/><br/>10. SPECTROPHOTOMETRY <br/><br/>Introduction <br/><br/>History and Spectrophotometry <br/><br/>Spectrophotometer - Definition <br/><br/>Spectrophotometer - Components <br/><br/>Spectrophotometer - Design of the Device <br/><br/>Spectrophotometer - Basic Principle <br/><br/>Using Spectronic-20 Spectrophotometer <br/><br/>Spectrophotometers - Different Types <br/><br/>Spectrophotometer - Source of Light <br/><br/>Spectrophotometer - Methods of Measurement <br/><br/>Spectrophotometer - Uses <br/><br/>Spectrophotometer - Applications <br/><br/>Colorimeter and Spectrophotometer - Differences <br/><br/>11. COLORIMETRY<br/><br/>Introduction <br/><br/>History and Colour/Colorimetry <br/><br/>Colorimetry - Definition <br/><br/>Colorimeter - The Instrument <br/><br/>Principle of Colorimetry <br/><br/>Chemistry of Colorimetry <br/><br/>Colorimeter - Operational Procedure (Experimental Method) <br/><br/>Colorimetry - Chemical Method<br/><br/>Absorption Colorimeter <br/><br/>Photo-Electric Colorimeter - Operation <br/><br/>Colorimetric Assays <br/><br/>Precautions to be taken in Colorimetry <br/><br/>Factors that affect/influence Colorimetric Measurements <br/><br/>Advantages and Disadvantages of a Colorimeter <br/><br/>Uses of Colorimeter <br/><br/>Colorimetry - Applications in Plant Sciences <br/><br/>Colorimetry - Applications in Other Fields <br/><br/>12. HYDROGEN-ION CONCENTRATION (pH) <br/><br/>Introduction <br/><br/>Earliest pH Meter - Litmus <br/><br/>Hydrogen Ion Concentration (pH) <br/><br/>Measurement of pH <br/><br/>pH Meter <br/><br/>pH Meters - Types <br/><br/>Accurate Measurement of pH <br/><br/>pH Indicators <br/><br/>Buffers and Buffer Solutions <br/><br/>Determination of pH <br/><br/>Care and Use of pH Meter <br/><br/>pH Electrode / pH Meter <br/><br/>pH Scale <br/><br/>Uses of pH Meters <br/><br/>pH Meters - Advantages and Disadvantages <br/><br/>pH Controller <br/><br/>Calibration of pH Meter - Why? <br/><br/>Care of pH Electrodes <br/><br/>pH Measurement - Applications <br/><br/>pH in Nature <br/><br/>pH and Life <br/><br/>xiv Research Methodology in Plant Science<br/><br/>13. MICROTOMY AND MICROTOME <br/><br/>Introduction <br/><br/>History and Microtomy <br/><br/>Parts of a Microtome <br/><br/>Proposed Classification (Types) of Microtomes <br/><br/>Microtome - Knife Design and Cut Types <br/><br/>Sharpening of the Knives <br/><br/>Method of Sectioning by a Microtome <br/><br/>Maintenance of a Microtome <br/><br/>Applications of a Microtome<br/><br/>Conclusion <br/><br/>14. BIOSTATISTICS <br/><br/>Introduction <br/><br/>History and Biostatistics <br/><br/>Scope of Biostatistics <br/><br/>Principles of Experimental Design and Statistical Inference <br/><br/>Tabular Design <br/><br/>Tabular Presentation of the Raw Data<br/><br/>Tabular Presentation of Data <br/><br/>Types of Tables <br/><br/>Best Practice of Presenting the Table <br/><br/>Graphical Design <br/><br/>Diagrammatic Representation of Data <br/><br/>Statistical Method - Types <br/><br/>Data (Information) - Types <br/><br/>Central Tendencies - Measures <br/><br/>Averages <br/><br/>Standard Deviation (SD) <br/><br/>Correlation Coefficient <br/><br/>Standard Error of Mean (SEM) and its Applications <br/><br/>Standard Deviation (SD) and Standard Error (SE) <br/><br/>Confidence Interval (CI) or Fiducial Limits <br/><br/>Distribution - Types <br/><br/>Null Hypothesisμ <br/><br/>Errors - Types <br/><br/>Level of Significance <br/><br/>Outliers <br/><br/>One - tailed and Two - tailed Test <br/><br/>Determination of Sample Size - Importance <br/><br/>Power of Study <br/><br/>Choosing an Appropriate Statistical Test <br/><br/>Common Problems Faced by the Researcher<br/><br/>Software for Biostatistics <br/><br/>Statistical Applications in Biological Research <br/><br/>Biostatistics - Applications <br/><br/>Recent Development in Modern Biostatistics <br/><br/>15. COMPUTERS IN LIFE SCIENCES<br/><br/>Introduction <br/><br/>Computer - Basic Principles <br/><br/>Computer - Parts <br/><br/>Computers - Types according to Size and Power <br/><br/>Brief History of Computing <br/><br/>Computers - Applications in Life Sciences <br/><br/>Computers in Agricultural Research <br/><br/>Computers and Plant Sciences <br/><br/>Importance of Computer Literacy and Useful Software for Students and Researchers in Life Sciences<br/><br/>16. APPLICATIONS OF RADIOISOTOPES IN BIOLOGY/PLANT SCIENCES<br/><br/>Introduction <br/><br/>Radioactive Isotopes <br/><br/>Half-Life (t1/2) <br/><br/>Half-life in Biology and Pharmacology <br/><br/>Radioactive Tracers <br/><br/>Tracer Studies / Tracer Techniques <br/><br/>Instruments to Measure Ionizing Radiation <br/><br/>Geiger-Muller Counter (GM Counter) <br/><br/>Scintillation Counter <br/><br/>Radioactivity - Applications in Life Sciences <br/><br/>Radioisotopes - Applications in Plant Sciences <br/><br/>Radioisotopes and Agriculture <br/><br/>Radioisotopes - Applications in Agriculture <br/><br/>Stable Isotopes - Use in Agriculture <br/><br/>Radioisotopes - Uses in Molecular and Cell Biology <br/><br/>Conclusion <br/><br/>17. BIOINFORMATICS <br/><br/>Introduction <br/><br/>Definition <br/><br/>History and Bioinformatics <br/><br/>Bioinformatics - Concept and Goal <br/><br/>xvi Research Methodology in Plant Science<br/><br/>Bioinformatics - Relevance to Biotechnology <br/><br/>Bioinformatics - Important Thrust Areas <br/><br/>Bioinformatics - Uses <br/><br/>Goals of Bioinformatics <br/><br/>Approaches of Bioinformatics <br/><br/>Bioinformatics in relation to other fields <br/><br/>Conclusion <br/><br/>18. POLYMERASE CHAIN REACTION (PCR) <br/><br/>Introduction <br/><br/>PCR - What it is?<br/><br/>PCR - Principle <br/><br/>PCR - General Protocol <br/><br/>PCR - Different Schemes and Types <br/><br/>Applications of PCR in Different Disciplines <br/><br/>PCR - Benefits, Drawbacks and Limitations <br/><br/>Conclusion <br/><br/>19. PHOTON COUNTING AND PHOTO-MULTIPLIER TUBES (PMTs) <br/><br/>Introduction <br/><br/>Photon Counting <br/><br/>Photomultiplier Tube (PMT)<br/><br/>Photomultipliers - Properties <br/><br/>Photomultipliers - Modification <br/><br/>Photon Counting: SPCMs, CPMs and CCDs: Which Technology Works Best? <br/><br/>The 3 - Technologies <br/><br/>SPCMs (Single Photon Counting Modules) <br/><br/>CPMs (Channel Photo Multipliers) <br/><br/>CCDs (Charged Coupled Devices) <br/><br/>Photon Counting Mode (Analog and Digital) <br/><br/>Photon Counting Methods <br/><br/>Detectors for High Speed Photon Counting <br/><br/>Photon Counting Techniques <br/><br/>Detector Principles <br/><br/>Photon Detection - Uses <br/><br/>Photon Counting - Applications <br/><br/>Photon Counting - Benefits <br/><br/>Conclusion <br/><br/>REFERENCES
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Varalakshmi, D
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name	Pullaiah, T.
942 ## - ADDED ENTRY ELEMENTS (KOHA)
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Source of classification or shelving scheme	Not for loan	Collection code	bill no.	bill date	Home library	Current library	Date acquired	Source of acquisition	Coded location qualifier	Cost, normal purchase price	Full call number	Accession No	Date last seen	Price effective from	Koha item type	Public note
Dewey Decimal Classification	Not For Loan	Agricultur	NIPA/21-22/6196	30.03.2021	CENTRAL LIBRARY	CENTRAL LIBRARY	20/10/2021	New India Publishing Agency, New Delhi	REF	425.00	574.028 NAR	36398	20/10/2021	20/10/2021	BOOKS	Reference Books
Dewey Decimal Classification		Agricultur	NIPA/21-22/6196	30.03.2021	CENTRAL LIBRARY	CENTRAL LIBRARY	20/10/2021	New India Publishing Agency, New Delhi		425.00	574.028 NAR	36399	20/10/2021	20/10/2021	BOOKS
Dewey Decimal Classification		Agricultur	NIPA/21-22/6196	30.03.2021	CENTRAL LIBRARY	CENTRAL LIBRARY	20/10/2021	New India Publishing Agency, New Delhi		425.00	574.028 NAR	36400	20/10/2021	20/10/2021	BOOKS