Physiology Practical Manual

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Contents
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HAEMATOLOGY EXPERIMENTS
To skily the vampend wice and intesting det under how power and high power
To stunty harmocytometer and to enflect blond cumple
14 Determination of re red bhood cell (IBC) count
Determination of hital leukocyte count (TLC)
15 Extinsation vif haemoglobin by Sahlr's method
Preparation of blood smear (nins) and identification of various cells To do the differential leukocyte count (DLC) of your own blood
To determine bleeding time (BT) and clotting time (CT) of your own blood
10 Determine your own blood group
1.10 Determination of erythrocyte sedimentation rate (ESR) (Demonstration)
1.11 Determination of packed cell volume (PCV) and calculation of blood indices
1.12a Determination of specific gravity of a given sample of blood by copper sulphate method (Demonstration)
1.12h. To study the effect of hypotonic, hypertonic, isotonic saline. HCI and tannic acid
1.12c. Determination of osmotic fragility of erythrocytes
1.13. Determination of reticulocyte count (Demonstration)
1.14. Determination of platelets count
1.15. Determination of absolute eosinophil count (Demonstration)
Section 2: AMPHIBIAN EXPERIMENTS
2.1 General apparatus used in the amphibian experiments
22 Demonstrate the following with gastrocnemius muscle and sciatic nerve preparation of frog
1. Recording of simple muscle twitch and effect of temperature on it.
II. Effect of strength of stimulus on muscle contraction.
III. Conduction velocity of sciatic nerve.
2.3. Demonstrate the following on gastrocnemius muscle and sciatic nerve preparation of frog.
I. Effect of two successive stimuli applied at different time intervals.
II. Genesis of tetanus.
2.4. Demonstrate the following on gastrocnemius muscle and sciatic nerve preparation
1. Effect of load on muscle performance.
II. Phenomenon of fatigue.
2.5. Demonstrations:
I. Recording of normal cardiogram of frog and to study effect of temperature on it
II. To study the effect of adrenaline, acetyl choline and atropine on frog's heart. III. To study effect of stimulation of vasosympathetic trunk and white crecentic line (Wcl)
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Phenomenon of myheart evrytale compensatory pause and refractory period in
Anchen a HUMAN EXPERIMENTS
M To ettich the phospesion of fatigue and the effect of various variables on it by To study the phervestitum of human by handgrip dynamometer
12
To reventabetonograph (EMG) Determination of conduction velocity of human ulnst nerve
35 56 Determination of blood pressure at rest in a vulunteer ("PY5.12) To minh the effect of postuiv on BP in human (PYS.12)
To study the effect of exer exercise on blood pressure (PY5:12)
Reconfing of body temperature
19. To anady the cardiovascular response to exposure of hand to cold 1.20 Determamation of physical fitness of a subject using Harvard step test
3.11. Recording of human electrocardiogram (ECG) 12. To study respiratory movements by stethograph
3.13 To study the effect of posture on vital capacity by vitalograph 3.14. Determination of various lung volumes and capacities by spirometry
3.15. Determination of basal metabolic rate (BMR) (Demonstration)
3.36. To determine energy cost of work and mechanical efficiency by using bicycle ergometer
(Demonstration) 3.37. To study human diuresis
3.18 To map the peripheral field of vision with perimeter (perimetry) (*PY10.20)
3.19 Mapping of physiologicai blind spot and calculation of optic disc size
3.20a. To test visual acuity ("PY10.20)
3.206. To test colour vision (*PY10.20)
3.21. Cardiopulmonary resuscitation: To demonstrate the technique of artificial respiration and cardiac resuscitation
3.22. General physical examination
3.23a Examination and recording of pulse ("PY5.12)
3.236 Clinical examination of cardiovascular system (CVS)
3.24 Clinical examination of respiratory system (*PY6.9)
3.25. Clinical examination of the abdomen
3.26. Clinical examination of sensory system (*PY10.11)
3.27. Clinical examination of motor system (*PY10.11)
3.28. To study superficial and deep reflexes (*PY10.11)
3.29. Clinical examination of cranial nerves (*PY10.11) (*PY10.20)
3.30. Clinical examination of higher functions of nervous system (*PY10.11)
3.31 Recording of electroencephalogram (EEG) 3:32 Recording of auditory and visual reaction time
3.33. Audiometry