Biochemistry and Clinical Pathology : Theory and Practical

Content
1. Scope of Biochemistry and Clinical Pathology in Pharmacy,
Cell and Biochemical Organization 1
2. Carbohydrates 16
3. Proteins and Amino Acids 26
4. Lipids 48
5. Nucleic Acids 58
6. Enzymes 69
7. Vitamins 83
8. Metabolism of Carbohydrates 93
9. Metabolism of Lipids 118
10. Metabolism of Amino Acids (Proteins) and Jaundice 128
11. Biological Oxidation 153
12. Minerals 163
13. Water and Electrolytes 175
14. Introduction to Biotechnology 180
15. Organ Function Tests and their Abnormalities 183
16. Pathology of Blood and Urine 189
17. Routes of Drug Administration 196
18. Coronavirus Disease 198
BIOCHEMISTRY PRACTICALS
Procedure of Separation of Plasma and Serum from Blood for Determination
of Various Constituents (Quantitative Analysis) 203
Experiment 1 Qualitative Analysis (Tests) of Carbohydrates 204
Experiment 2 Qualitative Analysis (Tests) of Proteins and Amino Acids 209
Experiment 3 Qualitative Analysis (Tests) of Lipids 213
Experiment 4 Urine Analysis (Normal and Abnormal Constituents) 218
Experiment 5(A) Determination of Glucose in Urine 226
Experiment 5(B) Determination of Creatinine in Urine 228
Experiment 5(C) Determination of Chlorides in Urine 230
Blood/Serum Experiments (Simulated/Assumption, i.e. Demonstration only)
Experiment 6(A) Determination of Creatinine in Blood/Serum 232
Experiment 6(B) Determination of Glucose in Blood/Serum 235
Experiment 6(C) Determination of Cholesterol in Blood/Serum 237
Experiment 6(D) Determination of Calcium in Blood/Serum 239
Experiment 6(E) Determination of Urea in Blood/Serum 241
Experiment 6(F) Determination of SGOT (AST) in Blood/Serum 243
Experiment 6(G) Determination of SGPT (ALT) in Blood/Serum 246
Experiment 7 Hydrolysis of Starch 248
Index 251