Lab Manual biochemistry

1.Commonly Used Laboratory Apparatus and Equipments Good Safe Laboratory Practice and Waste Disposal.
2. Preparation of Buffers and Estimation of pH.
. Urine Analysis to Estimate and Determine Normal and Abnormal Constans 5.
3. Chemical Components of Normal Urine. 4
Screening of Urine for Inborn Errors & Describe the Use of Paper Chem
6. Principles of Colorimetry
7a. Phlebotomy, Sample Collection and Discuss the Use of Anticoagulants
7b. Estimation of Serum Creatinine and Creatinine Clearance
8. Estimation of Serum Proteins, Albumin and A:G Ratio
9. Estimation of Serum Total Cholesterol and HDL Cholesterol
10. Estimation of Triglycerides
11. Estimation of Calcium and Phosphorous
12. Estimation of Serum Bilirubin
13. Estimation of AST or SGOT/SGPT or ALT
14. Estimation of Alkaline Phosphatase
15. Composition of CSF
16. Commonly Used Equipments/Techniques in Biochemistry Laboratory
7. Basis and Rationale of Biochemical Tests Done in the Disorder/Diseases Com
18. Principles of Spectrophotometry.
19. Basic Principles Involved in the Instruments Commonly Used in A Biochemin Laboratory and Their Applications.
20. Abnormal Constituents in Urine, Interpret the Findings and Correlate These with Pathological States.
Shill
AR
CHR
viii Lab Manual Practical Biochemistry
21. Estimation of Glucose, Creatinine, Urea and Total Protein in Serum.
22. Calculate Albumin: Globulin (Ag) Ratio And Creatinine Clearance
23. Calculate Energy Content tof Different Food Items, Identify Food Items with High and Low Glycemic Index and Explain the Importance of these in the Diet
24. Advantages and/or Disadvantages of Use of Unsaturated, Saturated and Trans Fats in Food.