Principles of Micribiology
- Delhi Swastik Pub. 2009
- 283p.
Contents 1. Introduction 1.1 Understanding Variations Nucleic Acids in Biosynthetic Variations 1.1.2 Variation Investigations. Easily Detected Variations. 1.2 Mutation 1.2.1 Mutations in Codons 1.3 Protein Synthesis by Substrate Constituents. 1.4 Relative Positions of Genes 1.5 Mating Types of Bacteria 1.6 Chromosome Mapping in Bacteria 1.7 Episomes 1.8 Cytoplasmic Inheritance in Microorganisms 1.9 Genetic Materials Replication and Recombinations. Chromosome Recombinants 1.12 Crossovers within Genes Methods of Microbiology 2.1 Characterization 2.1.1 Relation to Oxygen 2.1.2 Anaerobes as Normal Flora 2.1.3 Anaerobic Infections 2.2 Collection of Specimens 2.2.1 Selection of Specimens for Anaerobic Culture (0) Collection MA 2.2.3 Transport of Specimens 24.1 Nonselective Media 24.2 Use of Liquid Media 24.3 Selective Media 244 Inoculation Procedures 25 Anaerobic Holding Jar Procedure 2.6 Use of Anaerobic Systems 2.6.1 Anaerobe Jars 2.6.2 Anaerobic Glove Box 2.6.3 PRAS Media and the Roll-streak Tube Technique ests bre Sorgantom doctory m an empl diseases us in to and ents st are en h admi 23 Direct Examination of Speciinens 24 Selection and Use of Mediate es a 2.6.4 Other Anaerobic Systems progr med 2.7 Incubation 2.8 Examination of Anaerobic Cultures h pr 2.8.1 Morphologic Considerations gy DOS: 2.9 Microscopic features hic 2.10 Colonial characteristics ve 2.10.1 Determination of Relationship to Oxygen 2.10.2 Procedure for the Analysis of Nonvolatile Acids 2.10.3 GLC Standards and Controls 2.10.3 Equipment and Operating Conditions Biochemical Characteriation.. 2.11.1 Conventional Systems 2.11.2 Minitek and API 20A Packaged Microsystems 2.11.3 Presumpto Plate System 2.11.4 AT System 2.11.5 Microtube Plate Procedures 2.11.6 Rapid Enzyme Systems 2.12 Reporting of Results 2.12.1 Use of Reference Laboratories 3. Eukaryotic Microbiology 3.1 Malaria 3.1.2 Diagnosis of the Infecting Species 313 Identification Examination of the blood smear 3.2.1 Serologic Diagnosis 3.3 Babesiosis 3.3.1 Diagnosis 3.4 Leishmaniasis 3.4.1 Diagnosis 3.5 Trypansosmiasis 3.5.1 Chagas' Disease 3.5.2 African Trypanosomiasis 3.5.3 Diagnosis 3.6 Toxoplasmosis 3.7 Pneumocystis Infection 3.7.1 Collection and Handling 3.7.2 Stains Free-Living Pathogenic Amebae. 3.8.1 Morphology 3.8.2 Collection, Handling, and Storage of Specimens 3.8.3 Methods of Examination 3.8.4 Culture.. 3.8.5 Enflagellation Experiment 3.8.6 Other Culture Methods 3.8.7 Serology 3.9 Intestinal and Urogenital Protozoa 3.9.1 Laboratory Diagnosis 3.10 Amoebae. Entamoeba Histolytica Immunodiagnosis of Amebiasis 3.10.3 IHA Test 3.11 Flagellate Morphologic Identification of amebae Giardia Lamblia 3.11.2 Chilomastix Mesnili 3.11.3 Dientamoeba Fragilis (vill) 7.7 Isoenzymes (Isozymes) 7.8 How Enzymes Act. 7.8.1 Mechanism of Enzyme Action 7.9 Enzyme Induction 7.10 Enzyme Equilibria and Reversibility 7.11 Enzyme Control .... 7.11.1 "Feed-back" Controls 7.11.2 Energy Controls 7.12 Location of Enzymes in the Cell Metabolite Antagonism 7.12.1 Exoenzymes 7.12.2 Endoenzymes 7.13 Factors that Affect Enzymes 7.13.1 Enzyme Inhibitors Classification and Nomenclature of Enzymes Hydrolyzing Enzymes (Transferases) 7.15.2 Oxidizing and Reducing Enzymes (Oxidoreduc- tases) Electron-Transfer Oxidases Hydroperoxidases Adding and Removing Enzymes Nonoxidative Decarboxylases Hydrases and Dehydrases Isomerases Permease Enzymes Passive Transport Active Transpost 8. DNA Technology Plasmid Fingerprinting Reagents Equipment Procedure for Preparing Plasmid DNA Agarose Gel Electrophoresis 9. Food Microbiology Selection and Collection of Samples Handling Samples Preparing Sample and For Analysis Appropriate Tests Intepretation of Results