Detection and Diagnosis of Plant Diseases

Description:
The present book “Detection and Diagnosis of Plant Diseases” deals with actual practical trends in modern Plant Pathology. It furnishes protocol on recent advances in bio-chemicals, biotechnological methods and aims to cover many important aspects such as Plant Pathology, Microbiology, Agricultural Microbiology, Biochemistry and Molecular biology. This book is designed to need the practical requirement of graduate and post-graduate students studying Plant Pathology, Microbiology, Biotechnology and Biochemistry courses by providing a readymade solution to the most of common experiments prescribed by any Indian University. Beside the latest technological development given in the book can be of interest to researchers and scientists. Most attention is given to the principal and theory behind various protocols that are expanding in details to aid understanding. It contains fifteen chapters emphasized on good laboratory practices in introduction to Plant Pathology as well as Microbiological equipments, isolation of plant pathogens from plants samples and soil samples, evaluation of fungicide toxicity by various methods, plant diseases diagnosis; field and laboratory diagnosis and important serological and molecular techniques, important biochemical methods, preparation of buffer solutions and at last is various important information related to agriculture graduate and post graduate students.
Contents:
1.Instruments used in the study of plant diseases/pathogens
2.Methods to Prove Koch’s postulates
3.Preparation of culture media for the growth and isolation of fungi and bacteria
4.Isolation of plant pathogens
5.Staining of fungal pathogens
6.Staining of bacterial pathogens
7.Isolation and purification of plant pathogenic fungi from rhizosphere soil
8.Isolation and purification of plant pathogenic bacteria from rhizosphere soil
9.Plant disease diagnosis: the complete process of field observations, sample collection, sample packaging and laboratory diagnosis
10.Identification of Plant Parasitic Nematode-Heterodera
11.Identification of Plant Parasitic Nematode-Meloidogyne
12.Identification of Plant Parasitic Nematode-Anguina
13.Extraction of Nematode From Soil by Sieving and Decantation Method
14.Sampling and Extraction of Nematodes From Diseased Plant Materials
15.Preparation of Nematode Mounting
16.Preservation of disease samples by dry and wet methods
17.Evaluation of fungicide toxicity by food poisoning method against pathogens
18.Important serological and molecular techniques and instruments in Plant Pathology
Random Amplified Polymorphic DNA ( RAPD), PCR: Polymerase Chain Reaction
Enzyme Linked Immune Sorbent Assay (ELISA)
Dot- Immune Binding Assay (DIBA)
Immunosorbent Electron Microscopy (ISEM)
Isolation of fungal genomic DNA
Restriction Fragment Length Polymorphism (RFLP)
Amplified Fragment Length Polymorphism
Centrifuge, Spectrophotometer, PH meter, Microscopy (Phase Contrast Microscopy), Chromatography, Camera Lucida
19.Preparation of buffer and reagents
20.Methods of biochemical analyses
Estimation of Chlorophyll
Estimation of Proline
Estimation of Phenol
Estimation of Protein
Estimation of Total sugar (Reducing and Non-Reducing)
Estimation of Polyphenol Oxidase (PPO)
Estimation of Catalase (CAT) enzyme
Estimation of Phenyl-alanine Ammonia Lyase (PAL)
Estimation of Peroxidase (POD)
Estimation of Superoxide Dismutase (SOD)
21.General conversion tables and formulae