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020			_a978-93-86102-53-9
041			_aENGLISH
082			_a576 KUM
100			_aKumar, Vivek
245			_aLaboratory Manual of Microbiology
260			_aJodhpur _bScientific Publisher _c2019
300			_a215
500			_aDescription: This laboratory manual of microbiology has been written to meet the needs of students taking microbiology as major or subsidiary subject. The intention is to provide the students with well organized, user-friendly tool to better enable them to understand laboratory aspects of microbiology as well as to hopefully make learning laboratory material and preparing for independent player of a given experiment. Each exercise provides step-by-step procedure to complete the assignment successfully and easily. The lab exercises are designed to give the student hands-on laboratory experience to better reinforce certain topics discussed in exercise. The glossary is included covering terms as well as basic, discipline-specific terminology from microbiology that will be helpful to its readers. The main contents of the manual are: Microbiology laboratory practices and safety rules, Basic laboratory techniques, Microscopy, Staining and motility techniques, Environmental microbiology, Microbiological culture techniques, Growth of lactose fermenting and non fermenting microbes, Medical microbiology, Environmental effect on bacterial growth, Application of microbiology, Microbiology of milk and Appendices. The academic level of the book is graduate, post graduate students, research workers, teachers and scientists dealing with basic and applied aspects of microbiology. Contents: 1 Microbiology laboratory practices and safety rules I BASIC LABORATORY TECHNIQUES 2. Media preparation 2.1. Sub culturing or picking off: 2.2. Broth cultures 3. Sterilization and disinfection 4. Inoculation and other aseptic procedures 4.1. Using a wire loop 4.2. Flaming procedure 4.3. Using a pipette 4.4. Flaming the neck of bottles and test tubes 5. Inoculation using a pipette 6. Pouring the media in Petri plate 7. Use of spreader 8. Spread plate technique II MICROSCOPY 9. Use and care of microscope 10. Dark field microscopy 10.1. Bright field microscopy 10.2. Resolving power of microscope 10.3. Phase contrast field microscopy 10.4. Fluorescence microscopy 10.5. Electron microscopy 11. Ocular micrometery III STAINING AND MOTILITY TECHNIQUES 12. Staining preparations 13. Smear making 14. Simple staining 15. Gram staining of bacteria 16. Capsule staining of bacteria 17. Capsule staining by negative, indirect or background staining 18. Acid fast (Ziehl-Neelsen) staining of bacteria 19. Endospore staining of bacteria 20. Dorner’s method of endospore staining 21. Bacterial flagella staining 22. Viability staining technique of bacteria 23. Lactophenol cotton blue staining of fungi 24. Staining of Vesicular Arbuscular Mycorrhiza (VAM) 25. Staining of actinomycetes culture 26. Hanging drop slide for bacterial motility 27. Motility Determination (Tube method) 28. Slide technique for protozoa motility IV BIOCHEMICAL ACTIVITIES OF MICROBES 29. Urease activity of bacteria 30. Nitrate reduction test 31. Catalase test 32. Lipid hydrolysis test 33. Oxidase test 34. DNase production test 35. Starch hydrolysis test or amylase production test 36. Gelatin hydrolysis or production of gelatinase 37. IMVIC tests 37.1. Indole Test 37.2. Methyl Red and Voges-Proskauer Tests 37.3. Citrate Test 38. Hydrogen sulfide production and motility 39. TSI (Triple Sugar Iron) and KIA (Kligler's Iron Agar) 40. Casein hydrolysis 41. Estimation of phytohormone IAA production from bacteria 42. Estimation of ammonia production by bacteria 43. Solubilization of mineral phosphate by microbes V ENVIRONMENTAL MICROBIOLOGY 44. Multiple tube fermentation tests 44.1. Presumptive coliform test 44.2. Confirmed coliform test 44.3. Completed coliform test 45. Counting of Ascaris eggs in raw or treated sewage water 46. Determination of dissolved oxygen (DO) of water 47. Determination of biological oxygen demand (BOD) of water 48. Determination of chemical oxygen demand (COD) of water 49. Detection of coliforms in water using membrane filtration technique VI MICROBIOLOGAL CULTURE TECHNIQUES 50. Single Colony Isolation 51. Viable plate count or Pour plate or serial dilution agar technique 52. Counting of soil microorganisms 53. Isolation of Rhizobium from root nodules 54. Testing of nodulation ability by Rhizobia 55. Isolation of Azotobacter from soil 56. Carrier material for biofertilizers 57. Seed treatment with biofertilizers 58. Selection of bio-control agent 59. Isolation of pure culture and their preservation 60. Cultivation of anaerobic bacteria VII GROWTH OF LACTOSE FERMENTING AND NON- FERMENTING MICROBES 61. Growth of bacteria using selective and differential media 62. EMB (Eosine methylene blue) agar for E. coli 63. MacConkey agar for enterobacterieaceae 64. Salmonella-Shigella (SS) agar for Salmonella and Shigella 65. XLD (Xylose Lysine Deoxycholate) agar for enterobacteriaceae VIII MEDICAL MICROBIOLOGY 66. Micro-flora of upper respiratory tract 67. Enumeration of urine bacteria using pour plate technique 68. Use of urine dip slide for bacteria examination 69. Counting of bactiurea using calibrated loop method 70. Widal test: The tube agglutination test 71. Slide agglutination test for rapid diagnosis of pathogen 72. The Ouchterlony technique (double immuno-diffusion method) 73. Determination of Rh factor IX ENVIRONMENTAL EFFECT ON BACTERIAL GROWTH 74. Effect of pH on bacterial growth 75. Effect of oxygen on the growth of microorganisms 76. Effect of temperature on growth of microorganisms 77. Effect of osmotic pressure on growth of microorganisms 78. Effect of radiation (Ultraviolet light) on growth of microorganism X APPLICATION OF MICROBIOLOGY 79. Determination of viral plaque assay 80. The one step bacteriophage growth curve 81. Kirby-Bauer disk diffusion susceptibility test 82. Determination of phenol coefficient of a chemical or effect of disinfectant on bacteria 83. Determination of bacterial growth curve 84. Demonstration of Koch's postulates 85. Identifying Gram-negative enteric bacteria using API 20E system 86. Identifying Gram-negative, oxidase negative enteric bacteria using Enterotube II system XI MICROBIOLOGY OF MILK 87. Examination of milk quality by methylene blue reduction test 88. Counting of number of bacteria in milk using Breed method
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